human lung whole tissue lysate  (Novus Biologicals)

Journal: Heliyon

Article Title: Human intestine and placenta exhibit tissue-specific expression of RAGE isoforms.

doi: 10.1016/j.heliyon.2023.e18247

Figure Lengend Snippet: Fig. 5. RAGE immunoblotting analysis of human intestine. SDS-PAGE of lung lysate (1.5 μg/lane) as well as lysates from small intestine (SI) and colon (50 μg/lane) was performed under reducing conditions. Membranes were stained with three different anti-RAGE antibodies; SC A11 (A), AF1145 (B), and AB D01P (C). Membranes were also stained with the respective secondary antibodies only (negative control). GAPDH was used as a loading control. The uncropped images are shown in the supplementary material of the manuscript.

Article Snippet: Human Lung Whole Tissue Lysate (Adult Whole Normal) was obtained from Novus Biologicals and protein extracts from healthy human small intestine and colon were obtained from Santa Cruz Biotechnology.

Techniques: Western Blot, SDS Page, Staining, Negative Control, Control

Journal: Heliyon

Article Title: Human intestine and placenta exhibit tissue-specific expression of RAGE isoforms.

doi: 10.1016/j.heliyon.2023.e18247

Figure Lengend Snippet: Fig. 4. (A) RAGE immunoblotting analysis of human lung lysate. SDS-PAGE of lung lysate (1 μg/lane; PNGase F -) and deglycosylated lung lysate (1 μg/lane; PNGase F +) was performed under reducing conditions. Membranes were stained with four different anti-RAGE antibodies. Membranes were also stained with the respective secondary antibodies only (negative control). GAPDH was used as a loading control. (B) RAGE immunoblotting analysis of RAGE overexpressing (+) and control (−) HEK293T cells. SDS-PAGE was performed under reducing conditions. For WB analysis membranes were stained with anti-FLAG and anti-RAGE (AB D01P) antibodies. The uncropped images are shown in the supplementary material of the manuscript.

Article Snippet: Human Lung Whole Tissue Lysate (Adult Whole Normal) was obtained from Novus Biologicals and protein extracts from healthy human small intestine and colon were obtained from Santa Cruz Biotechnology.

Techniques: Western Blot, SDS Page, Staining, Negative Control, Control

Journal: Heliyon

Article Title: Human intestine and placenta exhibit tissue-specific expression of RAGE isoforms.

doi: 10.1016/j.heliyon.2023.e18247

Figure Lengend Snippet: Fig. 6. RAGE immunoblotting analysis of human placenta derived from different healthy (H) as well as GDM-, FGR- and PE-affected pregnancies. SDS-PAGE of lung lysate (1 μg/lane) as well as lysates from the placentas (25 μg/lane) was performed under reducing conditions. For WB analysis membranes were stained with three different anti-RAGE antibodies; SC A11 (A), AF1145 (B), and AB D01P (C). Membranes were also stained with the respective secondary antibodies only (negative control). Ponceau S staining was used as a loading control. The uncropped images are shown in the supplementary material of the manuscript.

Article Snippet: Human Lung Whole Tissue Lysate (Adult Whole Normal) was obtained from Novus Biologicals and protein extracts from healthy human small intestine and colon were obtained from Santa Cruz Biotechnology.

Techniques: Western Blot, Derivative Assay, SDS Page, Staining, Negative Control, Control

Journal: eLife

Article Title: Sperm-specific COX6B2 enhances oxidative phosphorylation, proliferation, and survival in human lung adenocarcinoma

doi: 10.7554/eLife.58108

Figure Lengend Snippet: ( A–C ) Kaplan-Meier curves for OS and FP in NSCLC ( A ) LUAD ( B ) and LUSC patients ( C ). ( D ) COX6B2 mRNA expression (RNA-seq RSEM, log2(norm count +1)) from TCGA Lung Cancer dataset. Bars represent median (normal: n = 110; tumor: n = 1017). P values calculated by Mann-Whitney test. ( E–F ) Whole tissue homogenates of LUAD tumors ( E ) and LUAD cell line lysates ( F ) were immunoblotted with indicated antibodies. Molecular weight (MW) markers are indicated. ( G ) IHC staining of non-malignant testis (a positive control), non-malignant lung (adjacent normal) and LUAD tissues. Scores ranged from 0 to 3. Scale bar, 50 μm. Bars represent mean ± SEM. p-Values calculated by Mann-Whitney test. ( H ) Representative confocal images of endogenous COX6B2 in HCC515 cells. Tom20 is used as a mitochondrial marker. Images were shown as Z-stack maximum projection from 0.3-μm-thick image. Scale bar, 10 μm.

Article Snippet: Human normal lung whole tissue lysate was purchased from Novus Biologicals (NB820-59237).

Techniques: Expressing, RNA Sequencing, MANN-WHITNEY, Molecular Weight, Immunohistochemistry, Positive Control, Marker